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KMID : 0861020080230010000
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Korea Journal of Herbology
2008 Volume.23 No. 1 p.0 ~ p.0
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Anti-inflammatory Effect of Evodia Officinalis DODE in Mouse Macrophage and Human Vascular Endotherial Cells
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Yun Hyun-Jeong
Heo Sook-Kyonug
Lee Young-Tae
Park Won-Hwan
Park Sun-Dong
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Abstract
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Objectives: Evodia officinalis DODE (EO), an herbal plant, has been widely used in traditional Korean
medicine for the treatment of vascular diseases such as hypertension. The crude extract of EO contains
phenolic compounds that are effective in protecting liver microsomes, hepatocytes, and erythrocytes
against oxidative damage. But EO has been little found to have an anti-inflammatory activity. We investigated
anti-inflammatory activity of EO in RAW 264.7 cells and human umbilical vein endothelial
cells (HUVECs).
Methods: Cytotoxic activity of EO on RAW 264.7 cells was investigated by using 5-(3-caroboxymethoxyphenyl)-
2H-tetra-zolium inner salt (MTS) assay. The nitric oxide (NO) production was measured by Griess reagent system. And proinflammatory cytokines were measured by ELISA kit. The levels of intracellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) expression were measured by flow cytometer.
Results: EO decreased LPS-induced NO production in RAW 264.7 cells. The inhibitory activity of EO on
LPS-induced NO release is probably associated with suppressing TNF-α, IL-6 and MCP-1 formation.
These results indicate that EO has potential as an anti-inflammatory agent. Moreover, EO decreased TNF-α-induced IL-8, IL-6 production, and ICAM-1 and VCAM-1 expression in HUVECs.
Conclusions: EO inhibits TNF-α-induced inflammation via decreasing cytokines production and adhesion
molecules expression. These results indicate that EO has potential as an anti-inflammation and antiartherosclerosis
agent.
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KEYWORD
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Evodia officinalis DODE, anti-inflammatory activity, cytokine, adhesion molecule
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